Screening seed lots before movement
Seed lot testing supports seed health assessment, phytosanitary certification and export certification by identifying potential pathogen presence before distribution or international movement.
:quality(75))
Featured Resources
Featured Resources
:quality(75))
:quality(75))
:quality(75))
Why choose LGC for crop disease detection
Supporting agricultural genomics and crop pathogen detection programmes with Biosearch Technologies nucleic acid extraction, PCR, KASP™ genotyping and sequencing solutions.
:quality(75))
Global Reach
144
Countries served through LGC's global network, supporting customers across diagnostics, genomics and agricultural testing applications.
Customer community
37,000+
Customers supported with solutions for diagnostics, genomics and life science workflows.
Frequently asked questions
How do you detect pathogens in seed lots before symptoms appear?
PCR and qPCR-based methods support sensitive nucleic acid detection for pathogen identification in seed materials before visible symptoms develop.
Can assays run directly on crude plant extract?
Some workflows may utilise crude plant extracts; however, assay performance can be influenced by sample quality and the presence of inhibitory compounds.
How do you deal with PCR inhibitors in plant tissue?
Polyphenols and polysaccharides found in plant tissues can interfere with amplification. Appropriate sample preparation methods and assay chemistries can help reduce inhibitory effects.
How many pathogens can be multiplexed in a single reaction?
The number of targets depends on assay design and instrument capability. Multiplex PCR is commonly used when several pathogen targets need to be screened simultaneously.
Should we use a probe-based or dye-based assay for plant pathogens?
The choice depends on testing objectives. Probe-based assays are typically selected when high target specificity is required.
When is sequencing a better choice than PCR for crop screening?
Sequencing approaches may be useful when broader pathogen investigation or pathogen surveillance is required beyond known targets.
Can you design a custom assay for a specific pathogen?
LGC can support discussions around assay development and technology selection for pathogen screening applications.
Do you supply assays suitable for phytosanitary or export testing?
Testing requirements vary by programme and target pathogen. Screening objectives can be discussed with the technical team to identify suitable approaches.
Can screening workflows be customised for specific crop-pathogen combinations?
Custom assay development, optimisation and validation strategies can support targeted detection of specific plant pathogens across different crop species, sample types and screening objectives.
How can nucleic acids be extracted from plant samples for pathogen testing?
Plant pathogen testing typically begins with nucleic acid extraction to recover pathogen DNA or RNA while minimising inhibitory compounds present in plant tissues. Magnetic bead-based purification methods, such as sbeadex® extraction chemistry, can support consistent nucleic acid recovery for downstream PCR, qPCR and sequencing workflows.
:quality(65))
Need help with crop pathogen detection?
Whether you are screening seed lots, investigating latent infections, supporting phytosanitary testing or developing a pathogen detection workflow, our experts can help you identify the right molecular technologies for your programme.