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Custom Oligo Manufacturing and In-House Synthesis Solutions

Biosearch Technologies supports oligonucleotide production in two ways: through custom oligo synthesis services and through the instruments, reagents and consumables required for in-house oligo synthesis. Whether you require oligonucleotides manufactured to your specifications or want greater control through internal production, our expertise spans the oligo synthesis workflow. We manufacture custom primers, probes, NGS adapters and modified oligonucleotides, while also supplying oligo synthesizers, phosphoramidites, solid supports, fluorophores and quencher chemistries for in-house production.

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Laboratory personnel operating automated synthesis equipment and preparing reagents within a controlled manufacturing environment for oligonucleotide production.

Support for every stage of oligo production

From assay design and optimisation to commercial manufacturing

Molecular assay performance depends on the quality, consistency and availability of the oligonucleotides at their core. Biosearch Technologies manufactures custom DNA and RNA oligonucleotides using automated solid-phase phosphoramidite chemistry for applications including PCR, qPCR, NGS, molecular diagnostics and nucleic acid therapeutics.

Synthetic oligonucleotides are assembled through cycles of detritylation, phosphoramidite coupling, capping and oxidation, enabling efficient synthesis of complex, highly modified sequences with per-cycle coupling efficiencies typically exceeding 99%.

Unlike suppliers that focus solely on oligo manufacturing, Biosearch Technologies supports the entire synthesis ecosystem. We manufacture custom oligonucleotides, develop oligo synthesizers, and produce key synthesis components including phosphoramidites, CPG solid supports, fluorophores and BHQâ„¢ quencher technologies. We provide enhanced supply-chain security, manufacturing control and scalable production for customers to source either finished oligos or the tools required to produce them internally.

Explore custom oligo servicesExplore solutions for in-house synthesis

Oligo synthesis solutions for molecular diagnostic and therapeutic programmes

Whether you are developing a new assay, scaling commercial manufacturing, specifying advanced oligonucleotide modifications or securing supply continuity, LGC provides the expertise, manufacturing capabilities and quality systems needed to support your programme.

Assay developers

Developing PCR, qPCR and NGS assays that require high-quality oligonucleotides, specialised modifications and consistent manufacturing to support analytical performance and reproducibility.

Molecular scientists

Designing primers, probes and sequencing oligos for demanding applications where sequence quality, purity and modification chemistry can directly influence assay sensitivity and specificity.

NGS and genomics teams

Seeking reliable manufacturing of indexing primers, adapters and custom oligos to support sequencing workflows, assay development and validation programmes.

How LGC supports oligo synthesis programmes

Developing and manufacturing high-performance oligonucleotides requires expertise in nucleic acid chemistry, scalable manufacturing and quality-driven production. LGC supports organisations whether they choose to outsource oligo production or establish in-house synthesis capabilities. Our portfolio spans custom oligo manufacturing, MerMadeâ„¢ oligo synthesizers, phosphoramidites, solid supports, fluorophores, quenchers and technical expertise. Customers can engage with a single supplier across the full oligonucleotide synthesis workflow, from raw materials through to commercial-scale supply.

Who we support

LGC supports organisations across the molecular diagnostics, genomics and life sciences sectors, including diagnostic manufacturers, assay developers, genomics laboratories, contract research organisations, academic institutions and biotechnology companies.

We also work with pharmaceutical and therapeutic developers that require access to scalable oligonucleotide reagents, specialised modification chemistries and reliable commercial supply to support product development, validation and commercialisation.

What they need

Organisations developing molecular assays and nucleic acid-based products require high-quality oligonucleotides with defined purity profiles, validated modification chemistries, scalable manufacturing processes and quality documentation capable of supporting assay development, analytical validation and commercial production.

To meet these needs, LGC provides custom oligos, primers, probes, NGS adapters, fluorophore and quencher technologies, phosphoramidites, solid supports and scalable manufacturing capabilities supported by ISO-certified quality systems and dedicated technical expertise.

Explore our oligo synthesis solutions

Automated oligonucleotide synthesis system with reagent bottles and fluidic connections used for DNA and RNA oligonucleotide manufacturing.

Custom Oligos, Probes and Primers

Design and manufacture custom DNA and RNA oligonucleotides, qPCR probes, hydrolysis probes, molecular beacons and specialised assay components with extensive modification, purification and scale options.

Custom Oligos, Probes and Primers
Automated oligonucleotide manufacturing system configured for high-throughput production of custom NGS indexing primers, sequencing adapters and specialty oligonucleotides.

NGS Indexing Primers and Adapters

Access custom indexing primers, sequencing adapters and NGS oligos manufactured to support assay development, validation and routine next-generation sequencing workflows.

NGS Indexing Primers and Adapters
Microscopic view of the porous structure of controlled pore glass (CPG), a solid support material used in oligonucleotide synthesis to enhance efficiency, reproducibility and product purity.

Solid Supports and Phosphoramidites for Oligo Synthesis

Source controlled pore glass (CPG) supports, phosphoramidites and specialised nucleic acid chemistry reagents designed to improve synthesis efficiency, reproducibility and product purity.

Solid Supports and Phosphoramidites for Oligo Synthesis
MerMade Pharos768 automated oligonucleotide synthesizer designed for scalable DNA and RNA oligo production with flexible throughput and efficient reagent utilization.

Oligo Synthesizers

Automate oligonucleotide production with scalable synthesizer platforms engineered for reliable synthesis performance, low reagent consumption and flexible throughput requirements.

Oligo Synthesizers

Explore related solutions

Technologies

Explore the technologies that underpin oligonucleotide design, synthesis, detection and commercial assay development.

Industries

See how LGC supports diagnostic manufacturers, biotechnology companies, pharmaceutical developers and research organisations requiring reliable oligonucleotide supply.

Industries

Related Products and Solutions

qPCR Probes

Black Hole Quencherâ„¢ (BHQâ„¢) Probes

Hydrolysis probes incorporating proprietary quencher chemistry for sensitive singleplex and multiplex real-time PCR assays.

Black Hole Quencherâ„¢ (BHQâ„¢) Probes

qPCR Probes

BHQplusâ„¢ Probes

Shorter probe designs engineered to improve mismatch discrimination and target specificity without compromising assay performance.

BHQplusâ„¢ Probes

qPCR Probes

BHQnovaâ„¢ Probes

Advanced double quenched hydrolysis probes designed to support challenging assay targets and demanding diagnostic workflows.

BHQnovaâ„¢ Probes

Probe Technologies

Locked Nucleic Acid (LNA) Probes

High-affinity probe designs that improve hybridisation specificity and mismatch discrimination for molecular detection assays.

Locked Nucleic Acid (LNA) Probes

NGS Oligos

NGS Indexing Primers and Adapters

Custom indexing primers and sequencing adapters manufactured for assay development, validation and production-scale NGS workflows.

NGS Indexing Primers and Adapters

Nucleic Acid Chemistry

PrimeMax CPGâ„¢ Solid Supports

Controlled pore glass solid supports engineered to optimise synthesis efficiency, reproducibility and purity in oligonucleotide manufacturing.

PrimeMax CPGâ„¢ Solid Supports

Oligo Synthesizers

MerMadeâ„¢ Oligo Synthesizers

Automated oligonucleotide synthesizer platforms designed for efficient, scalable and cost-effective DNA and RNA synthesis workflows.

MerMadeâ„¢ Oligo Synthesizers

Featured Resources

Sample Processing

Oligo Synthesis Masterclass

Webinar
Automated oligonucleotide synthesis system component used in the manufacture of custom oligos, probes and primers for molecular biology and diagnostic applications.

Oligos, Probes and Primers

Product Overview
Analytical laboratory systems supporting nucleic acid chemistry, oligonucleotide synthesis development and quality control processes.

Nucleic Acid Chemistry Reagents and Instruments

Product Overview
PCR tubes positioned on DNA sequence data representing software-guided primer and probe design for PCR and qPCR assay development.

PCR Forge Assay Design Software

Educational Resource
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Why choose LGC?

A trusted partner for custom oligo synthesis and commercial oligonucleotide manufacturing.

Deep nucleic acid chemistry expertise

More than 40 years of experience developing oligonucleotide synthesis chemistries, fluorophores, quenchers, solid supports and manufacturing processes for molecular biology, diagnostics and therapeutic applications.

Vertically integrated manufacturing

Unlike many oligonucleotide manufacturers, we produce key synthesis components including phosphoramidites, CPG supports, fluorophores and quencher technologies, helping ensure quality consistency, manufacturing control and supply continuity.

Commercial-scale supply security

Three global oligonucleotide manufacturing facilities operating within ISO-certified quality systems support business continuity, second-source strategies and long-term commercial supply requirements.

Controlled manufacturing environment supporting quality-assured oligonucleotide production and commercial-scale supply operations.

Understanding solid-phase oligonucleotide synthesis

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View case studies

Global reach

144

Countries served worldwide, supporting customers across diagnostics, genomics and life sciences.

Customers

37,000+

Organisations supported with scientific expertise, products and manufacturing solutions.

Frequently asked questions

What scale can you synthesise at?

LGC supports oligonucleotide production from nanomole research quantities through to gram-scale manufacturing for commercial diagnostic programmes.

What is your turnaround time?

Turnaround times vary depending on sequence complexity, modification requirements, purification specifications and manufacturing scale. Our technical teams can advise on timelines based on project requirements.

What modifications are available?

We offer a broad range of 5′, 3′ and internal modifications, including fluorophores, BHQ™ quenchers, LNA chemistries, phosphorothioate linkages, cholesterol conjugates, MGB chemistries and specialised phosphoramidites.

Which purification option should I choose?

Salt-Free (desalted) oligo purification is suitable for unlabelled oligos such as qPCR primers. For other applications involving unlabelled oligos, RPC would be beneficial as this removes contaminants such as truncated sequences, ammonium salts and impurities from the final product. HPLC based purification is recommended for more sensitive application requiring higher purity. RP-HPLC is useful for removing fluorescent contaminants, AX-HPLC is used to remove failure sequences that result from poor coupling during synthesis and Dual HPLC is AX-HPLC followed by RP-HPLC and is the method of choice when more stringent purification processes are needed. For NGS oligos used in library preparation, NxGradeâ„¢ optimised reversed-phase cartridge purification helps reduce the risk of cross-contamination.

Do you provide a certificate of analysis?

Yes. Quality documentation and supporting manufacturing records can be provided according to product grade and programme requirements.

Can LGC act as a second-source supplier?

Yes. Our global manufacturing network and vertically integrated production model support organisations seeking greater supply-chain resilience and business continuity.

How are custom oligonucleotides manufactured?

Custom oligonucleotides are typically produced using automated solid-phase phosphoramidite chemistry. During each synthesis cycle, the 5'-DMT protecting group is removed, a phosphoramidite is coupled to the growing oligonucleotide chain, unreacted sites are capped and the phosphite linkage is oxidised before the next nucleotide is incorporated. This process enables precise synthesis of DNA and RNA oligonucleotides with a wide range of modifications.

Looking for a custom oligo synthesis partner?

Whether you need custom primers and probes, NGS indexing oligos, specialised modifications or a long-term commercial manufacturing partner, our oligo synthesis experts can help.

Partner with LGC Biosearch Technologies to accelerate development, strengthen supply continuity and scale with confidence.

Explore custom oligo servicesExplore solutions for in-house synthesis