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PCR-based Genotyping for Agrigenomics

KASP™ (Kompetitive Allele Specific PCR) genotyping delivers accurate
bi-allelic discrimination of SNPs and InDels for plant and animal breeding. Designed for marker-assisted selection, it supports flexible, scalable and
cost-effective genotyping across agrigenomics workflows.

PCR workflow using automated pipetting for agrigenomics applications

PCR genotyping solutions for agrigenomics

PCR-based technologies for plant and
animal breeding

KASP™ is a PCR-based genotyping chemistry for accurate SNP and InDel analysis in plant and animal breeding programmes.

KASP enables reliable bi-allelic discrimination, helping breeders and researchers generate genetic data to support marker-assisted selection and trait development.

Its flexible assay design and scalable workflows support projects ranging from early-stage research to high-throughput breeding programmes.

Challenges facing modern breeding and
genotyping programmes

Breeding programmes often need to balance assay development, cost, throughput, compatibility and data quality requirements across large-scale genotyping workflows.

Converting markers into assays

Transforming published SNP and Indel markers into robust genotyping assays can require specialist design expertise.

Managing genotyping costs

Breeding programmes need affordable workflows that support large sample volumes without compromising data quality.

Scaling throughput

As programme sizes grow, laboratories need workflows that can scale efficiently from research to production.

Ensuring instrument compatibility

Integrating new genotyping chemistries with existing laboratory equipment can be a critical requirement.

Delivering reliable data

Accurate genotype calls are essential for informed marker-assisted selection and breeding decisions.

Accelerating breeding decisions

Breeders need rapid genotyping data to support selection decisions and shorten development timelines.

How LGC supports PCR genotyping for agrigenomics

Reliable genotyping programmes depend on more than assay chemistry. LGC combines proven KASP™ technology, assay design expertise and scalable workflow solutions to help breeding programmes generate actionable genetic data.

Who we support

Plant and animal breeding programmes, researchers and organisations implementing marker-assisted selection and SNP and Indel genotyping workflows.

What they need

Accurate SNP and Indel genotyping, flexible marker deployment, rapid assay development and scalable workflows that support marker-assisted selection across breeding programmes.

How PCR for agrigenomics connects

Technologies

PCR-based technologies supporting molecular detection, genotyping and breeding workflows.

Industries

See how LGC supports breeding programmes, agrigenomics laboratories and genomics researchers.

Industries

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Why organisations choose LGC

Supporting breeding programmes with trusted genotyping expertise

Proven genotyping expertise

KASP™ has supported PCR-based genotyping for more than 24 years, delivering reliable SNP and InDel analysis across diverse agrigenomics applications.

Established scientific credibility

Widely adopted by breeding and research organisations, KASP chemistry is supported by more than 2,500 peer-reviewed citations.

Flexible solutions for every stage

From custom assay design to high-throughput genotyping, LGC supports breeding programmes throughout the development pipeline.

KASP Reagents Guidance Notes

Explore resource

View case studies

Scientific impact

2500+

Peer-reviewed citations supporting KASP technology

Proven expertise

21+

Years supporting PCR-based genotyping programmes

Frequently asked questions

What is KASP genotyping?

KASP is a PCR-based genotyping technology used for bi-allelic discrimination of SNP and InDel markers.

Can KASP be used for marker-assisted selection?

Yes. KASP is widely used within plant and animal breeding programmes that rely on marker-assisted selection.

What plate formats are supported?

KASP workflows can be implemented in 96, 384 and 1536-well formats.

Which plate readers are compatible?

KASP assays are compatible with most FRET-capable plate readers and many qPCR instruments, helping laboratories integrate genotyping workflows into existing infrastructure.

Can I convert a published marker into a KASP assay?

Yes. KASP by Design services support the development of custom assays from published SNP and InDel markers.

What makes KASP a cost-effective genotyping solution?

KASP is designed to support low reaction volumes, flexible marker deployment and scalable workflows, helping breeding programmes manage genotyping costs while maintaining reliable results.

Talk to a genotyping specialist

Whether you are implementing marker-assisted selection, scaling a breeding programme or developing a custom SNP or Indel assay, our experts can help.