Custom DNA and RNA oligos for PCR, qPCR and diagnostics

Custom oligonucleotides can be tailored with specialised chemistries, labels and sequence modifications to support specific research, assay development and diagnostic requirements. Available as DNA oligos, RNA oligos, primers, probes and highly modified sequences, they can be configured to support PCR, qPCR, SNP genotyping, gene expression analysis and molecular detection workflows. Flexible design options allow researchers to optimise oligo performance for their target sequence and application.

Custom oligo synthesis for assay development

Custom oligo synthesis enables researchers and assay developers to design nucleic acid reagents tailored to specific assay requirements. Selection of sequence composition, probe chemistry, dyes, quenchers and modifications can help optimise assay sensitivity, specificity and reproducibility. These capabilities support projects ranging from early-stage research through to commercial molecular diagnostic development.

PCR and qPCR Primers for molecular detection

PCR and qPCR primers are critical components in nucleic acid amplification workflows. Well-designed primers help ensure efficient target amplification, accurate detection and reproducible results across a range of applications. Custom primers can be produced with standard or modified chemistries to support research, diagnostic and assay development requirements.

qPCR probe technologies for enhanced assay performance

Probe-based qPCR assays use fluorescently labelled oligonucleotides to provide highly specific target detection. Probe technologies such as BHQ™, BHQnova™, BHQplus™, MGB and LNA offer different performance characteristics depending on assay requirements. Selecting the most appropriate chemistry can improve signal-to-noise ratios, support SNP discrimination and enhance detection of challenging targets.

MGB and LNA probes for difficult targets

Minor Groove Binder (MGB) and Locked Nucleic Acid (LNA) probe technologies are widely used when additional specificity or mismatch discrimination is required. These probe formats can help distinguish closely related sequences, support SNP genotyping and improve assay performance for short or difficult target regions. Their enhanced binding properties make them valuable tools for demanding molecular detection applications.

BHQ probes and fluorescence-based detection

Black Hole Quencher™ probe technologies are designed to improve fluorescence quenching and minimise background signal in qPCR assays. Solutions including BHQ™, BHQnova™ and BHQplus™ probes support a broad range of assay designs and multiplexing requirements.

Modified oligos and custom oligonucleotide design

Custom oligos can be configured with fluorescent dyes, quenchers, ligands, modified bases and specialised chemistries to support specific assay requirements. These modifications allow assay developers to fine-tune probe behaviour, detection performance and multiplexing capabilities. Access to a broad range of modification options provides flexibility when designing assays for research and molecular diagnostic applications.

Molecular beacons and specialised probe formats

Molecular Beacons and Scorpions™ Primers incorporate specialised probe structures designed for real-time target detection. These formats provide alternative approaches to hydrolysis probe assays and can offer advantages in applications where assay specificity and signal discrimination are particularly important. They are commonly evaluated during assay development to identify the most suitable detection strategy for a given target.

Research-grade and commercial oligo manufacturing

Custom oligonucleotides are available for research, diagnostic and commercial applications, supporting projects from assay development through to routine production. Scalable manufacturing capabilities, quality-managed production processes and ISO 13485-managed workflows help organisations transition assays from development to commercial deployment while maintaining consistency, traceability and performance. As the original inventor of BHQ™ technology, Biosearch Technologies combines decades of oligonucleotide expertise with vertically integrated manufacturing and production facilities in the US and Europe, helping support quality, supply continuity and scalable commercialisation.