NGS adapters and indexing primers are specialised oligonucleotides used during NGS library preparation to enable sequencing and sample identification. Sequencing adapters provide the flow cell binding sequences and sequencing primer binding sites required for cluster generation and sequencing, while indexing primers introduce i5 and i7 indexes that allow multiple libraries to be pooled and analysed within a single run. Because multiplexed sequencing depends on accurate sample identification, adapter quality, oligonucleotide purity and contamination control are critical for maintaining sequencing accuracy, reducing sample misassignment and supporting confidence in downstream analysis.
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What are NGS adapters and indexing primers?
Essential oligonucleotides for sequencing and sample identification
Supporting NGS library preparation and multiplexed sequencing
Reliable sequencing results depend on more than adapter design alone. Researchers, sequencing facilities and commercial genomics organisations require high-purity oligonucleotides, robust contamination controls and flexible manufacturing support to meet the demands of modern sequencing workflows.
Who we support
Genomics researchers, molecular biology scientists, NGS core facilities, sequencing service providers and commercial genomics programmes requiring high-quality indexing primers and adapters for reliable library preparation and sequencing.
What they need
High-quality NGS adapters and indexing primers that combine purity, reproducibility, low cross-contamination and flexible delivery options to support multiplexed sequencing applications.
NGS adapters and indexing primers across applications and technologies
Applications
NGS adapters and indexing primers support next-generation sequencing workflows used in research, assay development and in vitro diagnostic applications.
Technologies
Sequencing adapters, indexing primers and multiplexing strategies support efficient library preparation, sample identification and high-throughput sequencing workflows.
Featured Resources
Featured Resources
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Why choose LGC for NGS adapters and indexing primers
Expertise and manufacturing capabilities for NGS workflows
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Customer base
37,000+
Customers supported globally
Global reach
144
Countries served worldwide
Frequently asked questions
What are adapters in Illumina sequencing?
Adapters are oligonucleotide sequences added to DNA or RNA fragments during NGS library preparation. They provide the sequences required for flow cell binding, sequencing primer attachment and sample indexing, enabling successful sequencing on platforms such as Illumina systems.
What is the role of adapters in DNA sequencing?
Sequencing adapters provide the functional sequences required for library preparation and sequencing workflows, including flow cell interaction, primer binding and sample indexing.
What is index hopping and how do I prevent it?
Index hopping is the incorrect assignment of sequencing reads to unintended index sequences. Using high-quality, low-contamination oligonucleotides and considering indexing strategies such as unique dual indexes can help reduce the risk of misassignment.
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Looking for NGS adapter and indexing primer expertise?
Whether you are establishing a new NGS library preparation workflow, scaling multiplexed sequencing programmes or sourcing custom index sets for large sequencing projects, our team can help identify the most appropriate adapter and indexing strategy for your application.