Solid-phase oligonucleotide synthesis relies on two essential elements: a solid support and reactive nucleotide building blocks. Controlled pore glass acts as the rigid matrix on which synthesis takes place, while nucleoside phosphoramidites are sequentially coupled to build the oligonucleotide chain. The choice of CPG pore size, loading capacity, support chemistry, and phosphoramidite design can influence synthesis efficiency, full-length product yield, impurity profiles, scalability, and manufacturing economics. Whether producing research oligos, diagnostic reagents, siRNA, or therapeutic ASOs, selecting appropriate supports and phosphoramidite chemistry is critical for achieving reliable synthesis performance.
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Optimising supports and chemistry for oligo synthesis
Core materials for solid-phase oligonucleotide synthesis
Supporting oligo manufacturing at every stage
Supporting oligonucleotide manufacturers, therapeutic developers and CDMOs with controlled pore glass, phosphoramidites, specialised modifications, nucleic acid chemistry reagents and scalable synthesis solutions across the oligonucleotide manufacturing workflow.
Who we support
Oligonucleotide manufacturers, CDMOs, process chemists, therapeutic developers, diagnostic assay developers and genomics organisations developing DNA, RNA, ASO and siRNA products.
What they need
Reliable controlled pore glass supports, nucleoside phosphoramidites, specialised modifications, scalable supply, lot-to-lot consistency and technical expertise that support efficient synthesis, scale-up and manufacturing performance.
How controlled pore glass and phosphoramidites connect
Applications
Support the synthesis of oligonucleotides used in molecular diagnostic assays, including PCR primers, probes, controls, sequencing workflows, and other nucleic acid-based testing applications.
Technologies
Controlled pore glass supports and phosphoramidite chemistry used to manufacture primers, probes and oligonucleotides for PCR, sequencing and molecular diagnostic workflows.
Featured Resources
Featured Resources
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Expertise in controlled pore glass and phosphoramidite manufacturing
Solutions designed to support oligonucleotide synthesis performance, consistency, and scale.
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Products
11,000
Products supporting global life science workflows
Global reach
144
Countries served worldwide
Frequently asked questions
How much does controlled pore glass cost?
Pricing depends on factors including pore size, loading specification, chemistry, scale requirements, and whether standard or customised supports are required. Contact our team to discuss bulk pricing and supply requirements.
Which pore size should I use for my oligo length?
Short oligonucleotides and typical siRNA strands are commonly synthesised using smaller pore sizes, while longer oligonucleotides often require larger pore sizes such as 2000-3000 Å to improve reagent accessibility during synthesis.
How does loading capacity affect yield?
Loading capacity influences the number of reactive sites available for synthesis. Excessive loading can increase coupling failures, while optimised loading can support improved synthesis efficiency and full-length product yield.
Should I choose CPG or polystyrene for oligonucleotide synthesis?
Controlled pore glass provides a rigid matrix with well-defined pore structures, while polystyrene resin offers alternative support properties. Selection depends on synthesis scale, sequence length, chemistry, and manufacturing objectives.
Which support suits ASO or siRNA synthesis?
Support selection depends on oligonucleotide length, modification profile, and process requirements. Therapeutic programmes often evaluate pore size, loading capacity, and chemistry based on the specific ASO or siRNA design.
What purity specification do you supply?
Purity specifications vary by product and application. Product-specific documentation is available upon request.
Do you supply at GMP grade and manufacturing scale?
LGC supports customers working from research through manufacturing-scale programmes, including therapeutic development applications.
How consistent is your material lot to lot?
Lot-to-lot consistency is a key consideration in manufacturing and process development. Materials are produced within quality-controlled manufacturing environments designed to support reproducible performance.
Is controlled pore glass available for GMP manufacturing?
Controlled pore glass requirements vary by application and manufacturing stage. LGC supports customers from research through manufacturing-scale oligonucleotide programmes and can discuss quality and supply requirements for specific projects.
What phosphoramidite modifications are available for therapeutic oligonucleotide development?
Biosearch Technologies offers a broad portfolio of phosphoramidite and solid-support modification chemistries, including GalNAc conjugation reagents, lipophilic modifiers and custom-designed chemistries that support ASO, siRNA and other nucleic acid therapeutic development programmes. These solutions can be incorporated into established oligonucleotide synthesis workflows to support targeted delivery and advanced oligonucleotide design strategies.
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Need support selecting controlled pore glass and phosphoramidites?
Whether you are optimising siRNA synthesis, scaling therapeutic oligonucleotide production, selecting the right pore size, or evaluating specialised phosphoramidite chemistry, our team can help identify solutions that fit your manufacturing and development objectives.