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Molecular Testing Technologies

Explore molecular technologies for nucleic acid amplification, quantitative analysis, quality measurement and genomic characterisation. Compare PCR, qPCR chemistries, quality measurement tools and sequencing workflows to identify the most appropriate approach for your analytical objectives.

Comparing molecular technologies for amplification, detection and genomic analysis.

Choose your technology

Select technologies based on workflow requirements, performance targets and analytical objectives.

Quality measurement and control

Monitor assay performance and verify analytical consistency throughout testing workflows.

Recommended for: Calibration verification, analytical measurement, assay monitoring and quality assurance.

Target amplification and detection

Amplify and detect DNA or RNA targets using established molecular workflows.

Recommended for: Target identification, molecular detection and qualitative testing applications.

Quantitative PCR analysis

Generate reproducible quantitative measurements using real-time PCR detection chemistries.

Recommended for: Gene expression studies, molecular quantification and assay optimisation.

qPCR detection chemistries

Compare sequence-specific probe detection with intercalating dye-based PCR chemistries.

Recommended for: Multiplex assays, method development and real-time PCR applications.

Targeted and genomic analysis

Compare targeted PCR workflows with high-throughput sequencing-based genomic analysis.

Recommended for: Variant analysis, genomic profiling and high-throughput sequencing studies.

Not sure which technology fits your workflow?

Our scientific specialists can help you select the most appropriate technology based on sensitivity, specificity, throughput and regulatory requirements.

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Why organisations choose LGC

Proven expertise across molecular workflows

Molecular expertise

Supporting nucleic acid testing, quality measurement and genomic analysis workflows.

Supporting quality, compliance and reliable performance.

Quality excellence

Products and services developed within established quality and regulatory frameworks.

Workflow integration

Technologies supporting efficient progression from assay development to routine testing.

Global reach

144

Countries served worldwide

Customer base

37,000+

Customers across sectors

PCR assay design simplified

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Frequently asked questions

How do probe-based and dye-based assays differ?

Probe-based assays use sequence-specific fluorescent probes to detect target DNA sequences, providing high specificity and enabling multiplexing. Dye-based assays rely on fluorescent dyes that bind to any double-stranded DNA generated during amplification. As a result, they differ primarily in how fluorescent signals are generated and how target sequences are detected.

When is PCR preferred over NGS?

PCR is typically used for rapid analysis of known targets, while NGS is better suited to comprehensive sequencing, variant discovery and broader genomic characterisation.

What factors should be considered when selecting a qPCR chemistry?

Assay specificity, multiplexing requirements, target abundance, workflow complexity and data quality requirements are key considerations.

How do quality measurement tools support molecular testing?

QMT solutions help verify analytical performance, monitor assay consistency and support confidence in measurement results across laboratories.

How do NGS and GBS support high-throughput genomic analysis?

NGS and GBS workflows are designed for large-scale genomic studies requiring extensive sequence or marker information across multiple samples.

How are molecular technologies validated for regulated laboratory environments?

Validation typically includes assessment of accuracy, precision, specificity, sensitivity, reproducibility and fitness for the intended application, supported by documented quality processes.

Find the right technology

Whether you are developing diagnostic assays, implementing quality control programmes or advancing genomic research, our scientific experts can help identify the most appropriate technology for your workflow and performance requirements.