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Custom Oligos, Probes
and Primers

Custom oligos, probes and primers designed to support qPCR, SNP genotyping, gene expression and molecular diagnostics applications. Configure your oligos with a broad range of probe chemistries, dyes, quenchers and modifications.

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Custom oligonucleotide solutions for qPCR, SNP genotyping, gene expression and molecular diagnostics.

Which oligo modifications are right for
your assay?

Custom oligo chemistries, dyes and probe formats for assay optimisation

Custom oligo synthesis enables researchers and assay developers to tailor probe chemistries, labels and sequence modifications to meet specific assay performance requirements. They can be supplied as unlabelled primers, highly modified oligonucleotides, DNA/RNA hybrid sequences and specialised probe formats for PCR and qPCR applications.

Custom oligo synthesis provides the flexibility to incorporate the chemistries and modifications best suited to your assay, including fluorescent dyes, quenchers, minor groove binders (MGB), locked nucleic acids (LNA) and other specialised design elements. Available technologies include BHQ™ Probes, BHQnova™ Probes, BHQplus™ Probes, MGB Probes, LNA Probes, Molecular Beacons and Scorpions™ Primers, supporting applications ranging from gene expression analysis and SNP genotyping to molecular diagnostics.

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Supporting your assay development and oligo design

Researchers and assay developers often require flexible oligo synthesis solutions to address a range of performance, development and commercialisation requirements.

Broad probe technology choice

Select from BHQ™, BHQnova™, BHQplus™, MGB, LNA, Molecular Beacons and Scorpions™ Primers to identify the chemistry best suited to your assay requirements and performance goals.

Tailored to your application

Configure oligos with a wide range of fluorescent dyes, quenchers, modified bases and speciality modifications to support specific workflow and assay needs.

Enhanced performance for challenging targets

Access probe chemistries designed to support difficult sequences, including SNPs, A-T rich regions and targets requiring increased specificity and sensitivity.

Flexible from research to commercialisation

Support projects from early-stage research through to commercial molecular diagnostic programmes with access to research-grade and commercial-grade oligo manufacturing capabilities.

Extensive modification options

Take advantage of a broad portfolio of oligonucleotide modifications, fluorophores and quencher technologies to achieve your desired assay design and functionality.

Technical expertise and support

Work with experienced oligo synthesis specialists to help select appropriate probe chemistries and oligo designs for your application.

How LGC supports custom oligo synthesis

LGC Biosearch Technologies combines more than three decades of oligonucleotide synthesis expertise with innovative chemistry development and vertically integrated manufacturing capabilities. As the original inventor of BHQ™ technology, with manufacturing facilities in the US and Europe, Biosearch delivers consistent quality, supply reliability and scalable support for research, diagnostic and commercial workflows.

Who we support

Researchers, assay developers and commercial partners seeking proven oligo manufacturing expertise and trusted technical support.

What they need

High-quality custom oligos backed by BHQ™ innovation, global manufacturing capabilities and dependable supply chain continuity.

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Commercial-grade oligonucleotides manufactured within ISO 13485-certified quality systems for IVD assay development and commercial production. Scalable manufacturing and stringent quality controls support reliable molecular diagnostic workflows.

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Oligo Design Resources

Oligo Modifications

Explore a broad range of fluorescent dyes, quenchers, modified bases and speciality reagents available for custom oligo design.

Oligo Modifications

Applications, technologies and industries for custom oligos, probes and primers

Technologies

Probe chemistries and oligo technologies support PCR and qPCR assay development, detection and analysis.

Industries

See how LGC supports research organisations, assay developers and molecular diagnostics programmes with custom oligo synthesis expertise.

Industries

Featured Resources

Comparing probe technologies to optimise assay performance.

How to Choose Between LNA, MGB and BHQplus Probes

Blog
Understanding LNA chemistry and its role in assay performance.

Locked Nucleic Acids (LNA): Understanding the Technology

White Paper
Understanding quencher technologies for improved assay performance.

Understanding Black Hole Quencher (BHQ™) Dyes

Blog
Exploring probe-based detection strategies for real-time PCR.

Molecular Beacons for Real-Time PCR Detection

Blog
View all resources

Why organisations choose LGC

Supporting research, assay development and molecular diagnostics with flexible custom oligo synthesis solutions.

Extensive probe chemistry expertise

Choose from BHQ™, BHQnova™, BHQplus™, MGB, LNA, Molecular Beacon and Scorpions™ technologies to support a wide range of assay requirements.

Flexible customisation options

Build oligos to your exact specifications with access to fluorescent dyes, quenchers, modified bases and speciality modifications.

From research to commercial scale

Access scalable oligo manufacturing in the US and Europe, supported by quality-managed production processes and supply chain continuity.

Choosing the best qPCR probe chemistry for your assay

View guide

View case studies

Global reach

144

Countries served worldwide

Customer base

37,000+

Customers supported worldwide

Frequently asked questions

Which probe chemistry is best for SNP genotyping?

MGB, LNA and BHQplus probe chemistries are commonly used for SNP detection because they offer enhanced duplex stability, specificity and mismatch discrimination.

When should I use an LNA probe?

LNA probes are well suited to applications requiring high specificity and precise control of probe melting temperature.

What are the advantages of BHQnova probes?

BHQnova probes improve quenching efficiency and reduce background signal in longer probe designs, helping increase sensitivity.

Can custom oligos be manufactured for commercial diagnostic applications?

Yes. Commercial-grade oligos are available through ISO 13485-managed manufacturing processes designed to support molecular diagnostic programmes.

What types of modifications are available?

Available modifications include fluorescent dyes, Black Hole Quenchers™ (BHQ™), amino and thiol groups, ligands, modified bases, MGB chemistries, LNA chemistries and other specialised reagents used to optimise assay performance.

Talk to an oligo synthesis specialist

Whether you are developing a new assay, optimising probe performance or scaling towards commercial production, our experts can help identify the most suitable custom oligo, probe and primer solution for your project.

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